You know I adore you, right?
But still: if I catch you wasting cake, I'm going to have to bite you. :X
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That's kinky ʕ•ᴥ•ʔ
Dragons do bite during mating rituals you know?
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Thanks for the train! Have a bump :)
Also, enjoy the cake!
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happy cake day,
thanks for the train...
Biotechnology eh? nice. what did your thesis was on? (i assume master was with a thesis).
and it's always better to be with someone (cool and SG knowledgeable? keep him in your cellar, so he won't escape ;) )
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Cometabolic degradation of 4-chlorophenol in presence of vanillic acid as main growth substrate by Stenotrophomonas maltophilia KB2
I basically added bacteria, 4-chlorophenol (nasty stuff) and vanillic acid to a bottle and check how bacteria biodegrade it. So it was biochemistry / microbiology thesis.
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nice.
4-chlorophenol does look nasty, but i'm used to that, working with pesticides ;).
but why vanilic acid? why not any other substrate? i mean, given the substrate and it's similarity to the vanillic acid the bacteria would only use it (degrade it) if it's more energy efficient.
aerobic or anaerobic degradation? i'm assuming anaerobic since you said bottle
i'm just wondering is all. i'm more into chemistry at the moment, but always had a passion for microbiology
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Scientists use mostly phenol, as it is pretty much identical in terms of structure, so bacteria are able to degrade it together with other chlorophenols. But phenol is toxic on it's own. So it has it's downsides (plus it's rather stinky xD). Like, what's certain strain good for if it's able to degrade 50 mg/L of 4-CP in presence of high concentrations of phenol. It's enough to have some spill and everything will be contaminated with it.
You can also use glucose, but it's inefficient. Bacteria will just say "OMG we have glucose, we don't want this stinky and toxic stuff". And degrade it firstly, then (maybe) move to 4-CP, but as they use constitutive enzymes to degrade glucose they don't produce ones involved in 4-CP degradation. Even if you induce bacteria with phenol prior to experiment, they will focus on glucose and loose ability to degrade 4-CP along the way.
So people at my uni knew that this strain is able to degrade BTEX compounds, and wanted to know if it's able to degrade 4-CP in cometabolic systems. That's why I used both phenol (to have some kind of reference to other scientific articles) and vanillic acid (VA), as it is somewhat similar to phenol, non-toxic and wide spread in environment.
And it was aerobic degradation, as this strain was isolated from sewage treatment plant. I used "bottle", as I didn't know how much do you know about all this stuff, and writing "conical flask with microbiology stopper" could make people wonder how it's supposed to look like.
So I cultivated bacteria on phenol to induce them and store like this up to 2 weeks. In conical flasks mixed basic salt medium with phenol / vanillic acid, with/ without 4-CP and see what happends. Inducing them at the beginning was enought for them to degrade 50 mg/L of 4-CP in less than 8 - 9 hours in systems with both vanillic acid and phenol. Degradation was slightly slower in presence of VA, but it was max 2h more. So pretty good.
And induced bacteria mixed with salt medium and 4-CP weren't able to degrade 4-CP. So VA was really helping them to "do their stuff", it wasn't artifact from prior induction.
And yeah, it's nasty. As it's main degradation product of some pesticides, which were widely used few years back.
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i know more about sewage treatment plant than "bottles" for the microbes ;)
nice work.
can i ask another question?
did you try to go back to another substrate (lets say OMG glucose) for 2 weeks than switch to 4-CP and see what happens? same strain, but maybe the degradation would be faster, or slower etc.
and btw, PhD is great, you should. but consider it's long, and the writing in the end is excruciating (i'm finishing mine now)
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I didn't use glucose, but if you'd add like one culture directly from culture dish (cultivated on nutrient agar) I think that result would be similar. It contains easy to use sources of carbon. So it wouldn't work, not without induction.
(Bacteria are normally cultivated on agar dishes with easy to use cources or carbon and energy. part of them are moved to systems with hard to degrade stuff just for time of experiments).
Tho I messed up at some point and forgot about my experiment before Christmas. So flasks were on their own for like two weeks? They didn't degrade 4-CP, but did degrade VA. (And it was in 4 centigrades, my other experiments were in 30 centigrades).
After adding them VA afterwards (to see if they will rise from dead like zombies) they degraded it, but didn't touch 4-CP at all. So they loose ability to degrade 4-CP along the way.
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i love it when a "mistake" turn out to be "planed".
could be the leftover enzyme and not viable bacteria? it's nice that they worked at 4C. not many do. especially the sewage treatment plant ones. usually used to higher temps
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Nope. They're intracellular enzymes (they aren't released to the medium), so when bacteria ate everything they could from medium they just digest these enzymes as well (proteins give similar amount of energy per 1 mol as hydrocarbons). It's like with humans, our organisms will use up hydrocarbons, fats and in the end proteins to keep us alive.
Also bacteria "amount" is measured using spectrofotometer at 600 nm. The higher value, the more bacteria there is. And it started to grow after adding VA.
So most probably it went like this:
Bacteria eat up whole VA and then digest unnecessary enzymes (which are used in degradation of VA). Then they started to use stuff from their reserves and when it was also gone they started to digest themselves and partialy went into semi-active mode. The weakest bacteria died, so they acted as energy source for other, still "alive" ones. And it was enough to keep them alive to the time I added them VA once again.
When I write it down like this, I see that I made them really nice hunger games in these conical flasks D:
And they did remove VA and part of 4-CP, though it took them more than 5 days vs few hours in 30 centigrades).
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hunger games ;)
and you got the famous lag from the growth model. that is also cool.
and now PhD? same field?
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Yeah, gonna try getting PhD but not in microbiology/biochemistry working with bacteria. More like basic research in new anti-cancer compounds, but most probably just in vitro tests.
I think it's gonna be interesting as well :D
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cancer is interesting.
plus, there is the added benefit of a "secure" funding... that is a big deal in a phd
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Happy belated Cake Day!:)
I hope that everything works out for you with your fellow Steamgifter.:) It's nice learning about people here sometimes.:)
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Happy Cakeday and thanks for a terrific train! Have a wonderful holiday too :)
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Nice cake. And good to hear you could use all 3 parts of the sentence. Don't lose it
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Happy cakeday!
Aww, i'm not passing the last two rules... Oh well...
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Happy Cake Day! 27th June is also birthday of someone important to me. :)
Have some evil cat and thank you for the train.
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Happy cakeday MsKotor!
May the cakes be with you xD
Also, why did you buy a pretty cake?
You can't eat it now :0
Well, still you better eat it.. Or I'll eat it in one gulp!
Don't start BL drama here
I hope the meaning of that BL isn't Boys Love :P
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Lol, didn't know about this BL acronym. The more you know...
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4 realz, it's here => www.steamgifts.com/giveaway/Fak3s/counter-strike-global-offensive
But seriously - It's my cakeday \o/
I registered on 27th June at 00:58. And now (here) is 27th June, ~22:00. So I have no idea why I don't have cake next to my avatar. Like you celebrate your bday after you're born, not before that. And SG reported that I had cakeday yesterday. Mostly probably bc of different time zones. And I planned everything for today, making most of GAs like week ago. So it's kind of weird :D
For the people who entered only for gamez. They're here, after SGtools check:
Train consist of 59 GAs, ~80 copies in total. Mostly my bundle leftovers that I had lying around:
But also 4 non-bundled games (yes, they're at very end of this train : ] )
For the people who can read:
It was a long ride. I registered when I was at the end of my bachelor degree to leech free gamez. But afterwards I noticed that I like to make GAs. It made me feel better when I was in shitty mood, and depressed. Like "yay, they liked the game I gave, it's so super cool!" stuff. So I stayed, and after some time I started to lurk in the forum more often to the point when I was sitting here most of the time after getting my master degree (in biotechnology if anyone is wondering). But since February I'm having less and less time, due to some stuff. That's main reason why I'm giving away pretty much all my spare keys, as I (most probably) won't be able to participate in future forum events.
(I also want to get promotion to 7th lvl after SSS, which is kinda hard after my LEGO Batman 3 GAs got nerfed and I "lost" 85 CV xD But I will get there in like a week. And I though that I will stay on lvl 5 forever xD Looks like not everything went accorfing to a plan : ] )
Why such requirements for GAs? I wanted to give to people who are active part of this community (and they give more than they take). To the people who most probably unknowingly helped me. I learned a lot by reading people who were discussing on various topics, were arguing or just trolling. I think it was really important experience for someone like me - ball of nerves with terrible social anxiety. But it's better now. Way better.
I also want to thank people, who talk with me from time to time on Steam, or even play in games with me. Though I know I wasn't really active lately, and I won't be active in like next month. Hope they are fine with it :D
And last thing, but not least important.
I have met someone, cool, here on SG. And I'd never thought that I will be able to use even one part of this sentence, not to mention all three at the same time. It's cool and makes me feel weird, but It's also something scary and unknown. Though I hope it's gonna end well. One way or another.
So have great holidays / free-time, fun during SSS and lot of cool time on SG forum ^_^
As always - please, don't start BL drama here, if you want just BL me back. I tagged people who I BL, so I don't enter into their GAs either way.
Also I brought a cake, tho I won't give it to you. It's nice cake, so it's only to look at it. Not cut it with knives D: (tho you can stab me instead, my scales are pretty hard so no much harm done that way)
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